CRYOPRESERVATION OF MURINE BONE MARROW CELLS AND THEIR TRANSPLANTATION / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-550133
ABSTRACT
Murine bone marrow cells were cryopreserved in liquid nitrogen with programmable freezing system as a source of hematopoietic cells to transplant into irradiated recipients for the treatment of hematopoietic failure. The results showed that CFU-GM in graft was slightly affected by freezing with a recovery of 75.7%, but CFU-S which act as major role in hematopoietic recons-titution was not obviously injuried (recovery of 126.7%). In the transplantation study, the frozen-thawed bone marrow cells gained survival rate of 43.6% which was slightly higher than obtained by fresh cells, suggesting that cryopreserved bone marrow cells still have intact hematopoietic reconstructive ability. For the purpose of preventing graft-versus-host disease (GVHD), chalone, an extract from calf spleen, was used in our studies and inhibited the early GVHD effectively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1985
Type:
Article
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