Gene Expression Regulation by Agonist-Independent Constitutive Signaling of Melanocortin-1 Receptor
Endocrinology and Metabolism
;
: 179-184, 2014.
Article
in English
| WPRIM
| ID: wpr-55020
ABSTRACT
BACKGROUND:
Melanocortin-1 receptor (Mc1r), a key signaling receptor for melanogenesis, has been reported to mediate migration of B16F10 melanoma cells. Interestingly, this activity appears to be a part of the constitutive signaling of Mc1r.METHODS:
We carried out small interfering RNA-mediated knock-down of Mc1r on murine melanoma B16F10 cells and performed microarray analysis to characterize changes in the gene expression profile.RESULTS:
We isolated 22 and four genes whose expression decreased and increased, respectively, by 2.5-fold or higher as the result of Mc1r knock-down. Several down-regulated genes have been proposed to be involved in cell migration. Among these genes are several members of the chemokine gene family.CONCLUSION:
We provide a gene set for further functional analyses of Mc1r. The Mc1r target genes we present may be particularly relevant for understanding the ligand-independent activity of Mc1r. Further examination of the mode of action may lead to novel strategies in regulating the migration and metastasis of melanoma cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Movement
/
Gene Expression Regulation
/
Genes, vif
/
Chemokines
/
Receptor, Melanocortin, Type 1
/
Microarray Analysis
/
Transcriptome
/
Melanoma
/
Neoplasm Metastasis
Limits:
Humans
Language:
English
Journal:
Endocrinology and Metabolism
Year:
2014
Type:
Article
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