Gene Expression Regulation by Agonist-Independent Constitutive Signaling of Melanocortin-1 Receptor
Endocrinology and Metabolism
; : 179-184, 2014.
Article
in En
| WPRIM
| ID: wpr-55020
Responsible library:
WPRO
ABSTRACT
BACKGROUND: Melanocortin-1 receptor (Mc1r), a key signaling receptor for melanogenesis, has been reported to mediate migration of B16F10 melanoma cells. Interestingly, this activity appears to be a part of the constitutive signaling of Mc1r. METHODS: We carried out small interfering RNA-mediated knock-down of Mc1r on murine melanoma B16F10 cells and performed microarray analysis to characterize changes in the gene expression profile. RESULTS: We isolated 22 and four genes whose expression decreased and increased, respectively, by 2.5-fold or higher as the result of Mc1r knock-down. Several down-regulated genes have been proposed to be involved in cell migration. Among these genes are several members of the chemokine gene family. CONCLUSION: We provide a gene set for further functional analyses of Mc1r. The Mc1r target genes we present may be particularly relevant for understanding the ligand-independent activity of Mc1r. Further examination of the mode of action may lead to novel strategies in regulating the migration and metastasis of melanoma cells.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Cell Movement
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Gene Expression Regulation
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Genes, vif
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Chemokines
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Receptor, Melanocortin, Type 1
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Microarray Analysis
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Transcriptome
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Melanoma
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Neoplasm Metastasis
Limits:
Humans
Language:
En
Journal:
Endocrinology and Metabolism
Year:
2014
Type:
Article