Effect of Estriol Sodium Succinate on Colony-Stimulating Factor Level in the Serum of Mice / 第二军医大学学报

ABSTRACT

We studied the changes of colony-stimu lating factor (CSF) level, the type of CSF after intraperi-toncal injection of estriol sodium succinate (E3?SNa) and the effect of E3?SNa itself on the proliferation of bone marrow precursors with the use of both agar colony assay and [3H]-TdR incorporation assay. Our experiment shows that CSF was at high level 6 h after E3?SNa administration and reached a peak by 24 h, and that elevation of CSF in the serum was maintained for at least 30 d. Using 0.14-28 mg of E3?SNa there was a dose-dependent increase in serum CSF when tested 24 h after E3?SNa treatment. The main type of CSF was GM-CSF. There was no direct stimulation of E32?SNa itself on the proliferation of mouse bone marrow precursors. So we think that the effect of estrogens on the hematopoiesis may, at least in part, be mediated by altered CSF activity.