THE GC ANALYSIS OF FATTY ACID IN HUMAN ADIPOSE TISSUE AND PLASMA / 营养学报

ABSTRACT

The total fatty acid in adipose tissue and total and free fatty acid in plasma, using tridecanoic (Cl30) and heptadecanoic acid (Cl70) as the internal standard (IS) respectively, were analysed by GC. In analysing total fatty acid, the esterification rate was 103.53% (IS Cl30) and 102.86% (IS C170); the recovery of lauric, myristic, stearic and arachidic acid was 101.0-103,3%, 102,1-494.1%, 103,1-104.5% and 100,0-105.0%; the CV of individual fatty acid were within the range of 2.24-8.46%. In analysing free fatty acid, the esterification rate in use of C17 0 as the IS was 102.99%; the recovery of lauric, myristic, stearic and arachidic acid was 83.5-87.3%, 96.4-99.6%, 94.3-101.9%, and 82.0-105.0% respectively, the CV of individual fatty acid were within the range of 1.41-6.21%. The procedure analysing total fatty acid is especially simple, speedy, and reliable.