Excitotoxic Cell Death in Cultured Retinal Neurons
Journal of the Korean Ophthalmological Society
;
: 1987-1999, 1997.
Article
in Korean
| WPRIM
| ID: wpr-55063
ABSTRACT
We examined excitotoxicity, putatively a major mechanism of ischemic neuronal death, in primary rat retinal cultures. Retinal cultures were prepared from newborn rats (day 1 or 2). Exposure of these cultures (DIV8-10)to NMDA or kainate induced neuronal death. Furthermore, MK-801 or CNQX each partially attenuated glutamateinduced neuronal death, suggesting that both NMDA and kainate receptors mediate it. Thy-1(+) retinal ganglion neurons, like neurons as a whole, were equally injured by NMDA and by kainate. However, GABA(+) or calbindin (+) neurons of the inner nuclear layer were resistant to NMDA, but highly vulnerable to kainate. These neurons may have AMPA/kainate receptors that are highly permeable to Ca2+, as they take up cobalt with kainate stimulation. These results suggest that the AMPA/kainate receptor, rater than the NMDA receptor, may mediate this pattern of selective neurnonal death.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Retinaldehyde
/
Dizocilpine Maleate
/
N-Methylaspartate
/
Cell Death
/
Cobalt
/
Receptors, Kainic Acid
/
6-Cyano-7-nitroquinoxaline-2,3-dione
/
Ganglion Cysts
/
Retinal Neurons
/
GABAergic Neurons
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
1997
Type:
Article
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