THIN LAYER CHROMATOGRAPHY ULTRAVIOLET DENSITO-METRIC DETERMINATION OF PUERARIN IN PLASMA & ITS PHARMACOKINETICS IN RABBITS / 中国药理学通报

ABSTRACT

A simple thin layer chromatography ultraviolet densitometric method has been established for the separation and quantitative determination of Puerarin in plasma. Using ethyl acetate metha-nol water toluene absolute alcohol ( 100 15.5 13.5 213.5 V/V ) as the developing system, 5?l supernatant of the sample extracts were spotted on high performance sillica gel G glass plates and developed. The separeted spots were scanned using Shimadzu CS-910 TLC scanner, the results were calculated by the areas of spots with Rf 0.47. The mininum detectable quantity of Puerarin was 7.5ng/spot. The plasma concentration-time curve of Puerarin in rabbits after single iv 30mg/kg was best fitted to a two- compartments open model. The T1/2? , T1/2? (3 were 5.7, 27.5 min respectively. This showed that Puerarin is distributed and eliminated in a fairly rapid rate in rabbits.