Comparative Studies on Kinetics of Mitochondrial LDH and Solubilized Mitochondrial LDH in Rat Liver / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1981.
Article
in Chinese
| WPRIM
| ID: wpr-550887
ABSTRACT
In this paper, we studied the kinetics of nitochondrial lactate dehydrogenase (LDH) and solubilized mitochondrial LDH in the rat liver. The apparent Km values of mitochondrial LDH and solubilized mitochondrial LDH for substrate pyruvate were 50.0 ?mol/L and 33.8 ?mol/L, and those for NADH were 35.3?mol/L and 21.4 ?mol/L, respectively. The apparent Km values of mitochondrial LDH were greater than those of solubilized nitochondrial LDH. The mitochondrial LDH in the rat liver was mainly LDH-5, which could be solubilized by 0.15 mol/L NaQ solution.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1981
Type:
Article
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