Isolation of epidermal desmosomes and preliminary analysis of desmosomal proteins / 第三军医大学学报

ABSTRACT

Desmosomes with well-preserved architecture were isloated from the epidermis of bovine muzzle.The thickness of the dense plaque was about 15 nm and the width of desmoso-mal interspace 30~40 nm.The midline in the desmosomal interspace was shown as a moderately electrondense material.The component proteins of the desmosomes were analyzed with SDS-PAGE.It was found that both DP Ⅰ and DP Ⅱ consisted of 3 polypeptides and the molecular weight of DP 1 a,b and c was 235 kd,226 kd and 215kd and that of DP Ⅱ a,b and c was 206 kd,198 kd and 186 kd respectively ; and DP Ⅲ was composed of 2 polypeptides and the molecular weight of DP I a and b was 87 kd and 86 kd.The above mentioned data were different from those of other reports,while the data of the other component proteins were similar to those of others.The molecular weight of DG I was 150 kd.that of DG Ⅱ a and b was 135 kd and 118 kd,that of DG Ⅲ was probably 22 kd.and that of DP Ⅳ was 82 kd.According to our findings,it is concluded that both DP Ⅰ and DP Ⅱ consist of 3 polypeptides and DP Ⅲ of 2 polypepties.