Inhibition effects of interferon-gamma and interferon-alpha on human retinal pigment epithelial cells in vitro / 眼科

ABSTRACT

PURPOSE. To evaluate the effects of interfe-ron-gamma (IFN-?) and interferon-alpha (IFN-?) on human retinal pigment epithelial (RPE) ceils in vitro. METHODS: Cultures of human RPE cells were incubated with either of the IFNs (10IU/ml～10~4 IU/ml) for 3～5 days, and proliferation rates of the cells were assayed by [~3H]-thymidine incorporation and liquid scintillation techniques. RESULTS: There was a slight inhibition on human RPE cells with dosages from 10IU/ml to 10~4IU/ml. The inhibition rates of IFN-? of 10~3IU/ml, 10~4IU/ml were 8%, 15%, 26%, and of IFN-? were 7%, 9%, and 16%, respectively. On the 5th day there was a slight decrease in the inhibition rate than on the 3rd day. CONCLUSION: IFN-? and IFN-? exerted a slight inhibition on human RPE cells lasting 3 days.