Inhibitory effect of IL-10 on induced expression of ICAM-l in renal tubular cells is mediated by down regulated activity of nuclear factor kB / 中华肾脏病杂志

ABSTRACT

Objecive To explore the effect of interleukin-10(IL10) on tumor necrosis factor-?(TNF?)- induced expression of intercellular adhesion molecule 1 (ICAM-1 )and related transcription pathway in human renal tubular cells. Methods Human renal proximal tubular cell line HK-2 cell was used as target cell. ICAM-1 was measured by cell ELISA and Northern blot in protein and mBNA levels. Activities of transcriptional factors nuclear factor GB(NFkB) and activator protein 1 (AP-1) were determined by electrophoresis mobility shift assay. Results TNF?-induced ICAM-l expression in HK-2 cells was increased in both protein and mNRA levels(P