Effects of anisodamine on the change of cytoskeleton of rat pulmonary microvascular endothelial cells induced by endotoxin / 中国药理学通报

ABSTRACT

AIM Observing the influence of lipopolysaccharide (LPS) on changes of the permeability and actin cytoskeleton of rat pulmonary microvascular endothelial cells (PMVEC) monolayer, we explore their relationship with ?-adrenoceptor(?ＡＲ) and G protein subunit(Gs?), and the interfering action of anisodamine. METHODS PMVEC was isolated and cultured from Wistar rat in vitro, radi- oligand binding assay and flow cytometer were used. RESULTS After incubation of LPS in vitro, the central F-actin of PMVEC depolymerized and its density decreased obviously , while the permeability of PMVEC monolyer increased significantly. Meanwhile it was found that LPS can induce the down regulation of 5-AR and Gsa protein level. Anisodamine can in- hibit these changes above. CONCLUSION LPS can directly cause the increase of permeability of PMVEC monolayer which relates to the depolymerization of central F-actin in PMVEC. Anisodamine may take part in regulating actin cytoskeleton of PMVEC and attenuate LPS-induced the increase of permeability of PMVEC monolaver by influencing two links of both ?-AR and Gsa protein