EFFECTS OF BURN SERA AND SUBESCHAR TISSUE FLUID ON APOPTOSIS OF POLYMORPHONUCLEAR LEUKOCYTES IN VITRO / 解放军医学杂志

ABSTRACT

To elucidate apoptosis of polymorphonuclear leukocytes (PMN) in early postburn stage and the effects of burn sera and subeschar tissue fluid (STF) on apoptosis of PMNs, 24 Wistar rats were divided into scald group and control group (no scald group) randomly. PMNs were harvested at 0, 12, 24h after scald injury,and percentage of apoptotic PMNs was measured by flow cytometry.Apoptosis of PMNs was again assayed with flow cytometer after being incubated with burn sera or STF harvested from scalded rats. The result showed that the percentage of apoptotic PMNs in scalded group were lower than that in control group,indicating burn sera and STF could inhibit apoptosis of PMNs in a dose dependent manner. It suggested that spontaneous apoptosis of PMNs is inhibited in early postburn stage, and burn sera and STF play an important role in this process.