THE PROTECTIVE EFFECTS OF CYCLOSPORINE A ON CONCANAVALIN A INDUCED LIVER INJURY IN KUNMING MICE / 解放军医学杂志

ABSTRACT

To investigate the possible effects of cyclosporine A(CsA) on the T cell dependent liver injury induced by Concanavalin A (Con A) in Kunming mice,25 Kunming mice were randomly divided into five groups. Con A (doses of 10mg/kg, 20mg/kg and 40mg/kg) was administered via the tail vein to duplicate the liver injury as the ConA group; CsA subcutaneously injected twice at doses of 130mg/kg 15 hours and 1 hour respectively before ConA challenge constitutes the CsA group. Phosphate buffered saline (PBS) only served as the control group. Blood samples for tumor necrosis factor alpha (TNF ?) and alanine aminotransferase (ALT) measurement were obtained at 8 hours after ConA administration. Histopathological examination was also performed for liver tissue. The results showed that ConA induced specific liver injury in Kunming mice was successfully duplicated. CsA markedly protected the mice from Con A induced liver injury by comparing the levels of serum ALT(44?16U/L vs 341?52U/L, P