Study on Improvement of Recovering Process of Synergier from Bacillus thuringiensis / 微生物学通报

ABSTRACT

Recovering process of synergier from Bacillus thuringiensis KN-11 was improved by membrance filter technique. The results showed that the synergier from ferment liquid could be fully intercepted by the nanomembrance (200 D), and reached the total recovery rate of 85.5% (0.1 ?m micromembrance, 10,000 D ultramembrance and 200 D nanomembrance). Comparing with traditional recovering technology, membrance filter recovering technology could raise content of synergier in condense liquid and powder (99.35 U/mL, 457.70 U/g ,respectively) ,get rid of most foreign substance (such as dextrose, nitrogen) and keep condense liquid and powder in good physical-chemical characteristics. The emulsion made by membrance filter recovering technology had many excellences in high activity ( 15,645-19,465 IU/?l ), low solid content and good flowing characteristics. The technical powder had high potency (100646 IU/mg).