Effects of lovastatin on the activation of NF-?B and the expression of tPA/PAI-1 of human tubular epithelial cells cultured in high glucose / 中华肾脏病杂志

ABSTRACT

Objective To observe the effects of high glucose(HG) on the expression of PAI-1 /tPA and the activation of NF-KB in human proximal tubular cell(HKC) ,and explore whether these effects can be reversed by lovastatin. Methods Chromogenic substance was used to show the activity of tPA and PAI-1. The expression of PAI-1 mRNA was examined by RT-PCR. Immunoblotting and laser confocal microscopy were applied to examine the expression of p65 in nuclear. Results HG could up-regulated the activity of PAI-1 from(8. 23?0. 02) to (8.40?0. 07) IU/ml, and down-regulated the activity of tPA from (6. 22?0. 52) to (4. 9?0. 11) IU/ml (control vs HG, P