PROTECTIVE EFFECT OF PYRROLIDINE DITHIOCARBAMATE(PDTC) ON ACUTE LUNG INJURY(ALI) INDUCED BY BLUNT CHEST TRAUMA COMBINED WITH LPS CHALLENGE IN RATS / 解放军医学杂志

ABSTRACT

To explore the protective effect of pyrrolidine dithiocarbamate (PDTC) on acute lung injury induced by blunt chest trauma combined LPS challenge in rats. The combined injury model was reproduced by blunt impact on the right lateral superior chest of rats with 350 kPa drive pressure inflicted with a small multi function bio impact apparatus, folloned by an injection of LPS (5mg/kg) into the fail vein. PDTC (120mg/kg) was injected intravenously fore injury in animals of the PDTC pretreatment group. The 48 hour mortality rate was recorded, myeloperoxidase (MPO), NF ?B activity, and TNF?, IL 8 mRNA expression and their proteins of lung tissue were examined. The results showed that PDTC obviously reduced the 48h mortality the activity of MPO and NF ?B, and the gene expression and protein crotein contents of TNF?and IL 8,suggesting that PDTC has a protective effect on the combined injury. This effect might be associated with its inhibitory effecton NF ?B activation and expression as well as the release of proinflammatory cytokines.