STUDY OF ACUTE LUNG INJURY AFTER LIVER ISCHEMIA REPERFUSION / 解放军医学杂志

ABSTRACT

To investigate whether liver ischemia and reperfusion directly affect functions of a remote organ (lung), we reproduced a model of hepatic lobar ischemia and reperfusion in rats. Twenty four healthy male SD rats were randomized into three groups (each group = 8) . Group A served as sham operated control group, group B was subjected to 90 min ischemia of a lobe of the liver, and group C animals underwent 120 min reperfusion, following 90 min ischemia of a liver lobe. In addition to histologic examination of the lung in each group, plasma tumor necrosis factor ?(TNF ?),interleukin 1?(IL 1?),alanine aminotransferase(ALT), aspartate aminotransferase (AST), lactate dehydrogenase(LDH) levels, total protein content in bronchoalveolar lavage fluid (BALF) and lung dry to wet weight ratio were respectively examined in A, B and C groups. Blood pressures were monitored in each group. Histological manifestations of damage to the lung occured in groups B and group C were characterized by obvious intra alveolar and interstitial edema with inflammatory cellular infiltration. Plasma ALT, AST, LDH, were significantly increased in group C animals ( P