ROLE OF pS_2 AND INTESTINA TREFOIL FACTOR(ITF) IN THE EARLY PHASE OF EPITHELIAL REPAIR OF STRESS-INDUCED GASTRIC LESION / 解放军医学杂志

ABSTRACT

To determine the role of pS 2 and ITF(both are members of trefoil peptides) in the early phase of epithelial repair of stress induced gastric lesion. Wistar rats were exposed to water immersion restraint stress(WRS) for 4h, gastric mucosal blood flow(GMBF) was measured with LDF 3 flowmeter, and the extent of gastric mucosal lesions was evaluated grossly and histologically. Expression of pS 2 and ITF mRNA was determined with RT PCR, and further examined with immunohistochemistry. The results showed that a single infliction of WRS produced numerous gastric mucosal erosions, but the number of these lesions gradually decreased and GMBF recovered at 2, 4, 8h after stress. The area of gastric mucosal lesion was reduced by 64 9% and GMBF being increased by 89 8% at 8h. Healing of stress induced ulcerations was accompanied by an increase in the expression of pS 2 (0 51?0 14 vs 0 77?0 11, P