THE ROLE OF NICOTINAMIDE ADENINE DINUCLEOTIDE HYDROGEN IN PREVENTING HUMAN HEPATIC CELL LINE L02 FROM APOPTOTIC DAMAGE AS A RESULT OF CHEMOTHERAPY / 解放军医学杂志

ABSTRACT

To investigate the role of NADH in preventing apoptotic damage of human hepatic cells induced by chemotherapy drug cisplatin, the ultrastructural changes in hepatic cells were examined with transmission and scanning electron microscopy, propidium iodide (PI) staining was used to measure the apoptotic rate with flow cytometry, the expressions of p53 and bcl 2 gene were detected with RT PCR, the change in caspase 3 and caspase 8 of apoptotic molecules was examined with colorimetric assay. The results showed that, compared with the group of cisplatin, morphological apoptotic changes were not obvious, the apoptosis rate was significantly decreased, p53 mRNA expression was decreased, bcl 2 mRNA expression was increased, caspase 3 and caspase 8 activity levels were kept in a low level in the group of NADH /DDP. The study indicates that NADH can prevent apoptosis of human hepatic cells, and increase the possibility of using NADH to reduce side effects of chemotherapy.