Evaluation on expression of recombinant S gene of human hepatitis B virus in vitro and in vivo / 中华传染病杂志
Chinese Journal of Infectious Diseases
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-552789
ABSTRACT
Objective To construct a DNA vaccine capable of expressing S gene of hepatitis B virus(HBV) and evaluate the expression of recombinant S gene in vitro and in vivo. Methods A cloned S X gene fragment was inserted into a eukaryote expression vector to construct a recombinant plasmid. The S gene was transcribed in vitro and expressed in a transfected cell line, and the efficiency of HBsAg in eliciting anti HBs was evaluated in mice. Results The expression of S gene was confirmed by Northern blotting, Western blotting, and ELISA(for both antigen and antibody detection). Conclusions The recombination and expression of S gene is achieved successfully in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Infectious Diseases
Year:
2000
Type:
Article
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