MODULATION OF ANTISENSE PHOSPHOROTHIOATE OLIGODEXYNUCLEOTIDES OF LAMININ RECEPTOR ON MMP-2、MMP-9 mRNA EXPRESSION IN HUMAN BILE DUCT CARCINOMA / 解放军医学杂志

ABSTRACT

This study was designed to investigate modulation of phosphorothioate oligodexynucleotides of laminin receptor on MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells. Treated with a concentration of 12?mol/L LNR AS OD in cuture medium, the relative MMP 2 and MMP 9 mRNA level at 72h in human bile duct carcinoma cells was evaluated by reverse transcriptase polymerase chain reaction. The results showed that the expression of MMP 2 and MMP 9 dropped obviously by AS OD group as compared with control group. The relative expression level of MMP 2 and MMP 9 gene mRNA decreased about 33 2% and 23 9% respectively. It is suggested that LNR AS OD is the regulator of MMP 2 and MMP 9 gene expression. It can decrease MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells. Understading the regulation of MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells might contribute to the development of a new preventive and theraputic strategy for tumor invasiveness.