ADHESION OF LYMPHOCYTES AND EOSINOPHILS IN PERIPHERAL BLOOD OF ASTHMA GUINEA PIG MODEL AND LUNG MICROVASCULAR ENDOTHELIAL CELLS / 解放军医学杂志

ABSTRACT

To explore the adhesion of peripheral blood lymphocytes and eosinophils and lung microvascular endothelial cells (LMEC) in asthma. Guinea pigs were divided into asthma model group and control group; the adhesion rates of isolated peripheral lymphocytes, eosinophils and the cultured LMEC monolayers were assayed.The mRNA expressions of VCAM 1 and eotaxin in LMEC were determined by RT PCR.The DNA binding activities of NF ?B and AP 1 of LMEC were detected by electrophoretic mobility shifts assay (EMSA). Under the stimulation with serum of asthma group①the adhesion rates of lymphocytes, activated eosinophils and LMEC were significantly elevated( P