DETECTION OF MITOCHONDRIAL MEMBRANE POTENTIAL CHANGES IN CARDIOMYOCYTES BY FLUORESCENT DYES JC-1 / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-553037
ABSTRACT
To explore the changes of mitochondrial membrane potential in rat cadiomyocyte apoptosis by fluorescent JC 1, H 2 O 2 was used to induce cadiomyocyte apoptosis, and JC 1 in combination with flow cyometry was used to detect the changes of mitochondrial membrane potential in the early stage of cadiomyocyte apoptosis. The results showed that living cardiomyocytes had a high mitochondrial membrane potential, JC 1 aggregates were formed in the inner membrane of mitochondria and emitted orange red fluorescence. H 2 O 2 caused the decrease of mitochondrial membrane potential, JC 1 aggregates were dissociated to monomer,which emitted green fluorenscence. So the red fluorescence decreased. It is suggested that JC 1 in combination with flow cyometry is an ideal method to detect the changes of mitochondrial membrane potential.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
2001
Type:
Article
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