Highly effective transformation of Cryptococcus neoformans / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-553307
ABSTRACT
Objective:
To establish a highly effective transformation method of Cryptococcus neoformans.Methods:
Special reagents was used to make C. neoformans take in external DNA under given condition. This chemical transformation result was compared with that of electrotransformation. The feasibility of this chemical transformation was tested by plasmid stability test.Results:
The efficiency of this chemical transformation was more than 103 transformants/?g plasmid DNA, far more than that of the traditional electrotransformation.Conclusion:
An appropriate transformation method is established for C. neoformans transformation, which has high transformation efficiency.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2000
Type:
Article
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