SCREENING AND CHARACTERIZATION OF HUMAN PHAGE ANTIBODY TO HEPATITIS C VIRUS E2 ANTIGEN / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)1982.
Article
in Chinese
| WPRIM
| ID: wpr-553469
ABSTRACT
To screen and characterize human phage antibody to hepatitis C virus E2 antigen. The recombinant phages were panned by HCV E2 antigen which was coated in a microwell plate. After three rounds of biopanning, 56 clones specific to HCV E2 antigen were obtained. The specificity of scFv was determined by ELISA method and cross reaction with BSA and competitive inhibition assay. HCV E2 phage antibody had a specific combination character with recombinant hepatitis C virus E2 antigen. The DNA sequence data showed that the scFv coding gene was 771bp in size
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
1982
Type:
Article
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