IN VIVO IMAGING OF TECHNETIUM-99m-LABELLED ANTIMYOSIN LIGHT CHAIN MONOCLONAL ANTIBODY (AMLCA) IN EXPERIMENTAL MYOCARDIAL INFARCTION IN DOGS / 解放军医学杂志

ABSTRACT

The biodistribution and the radio activity in blood clearance of 99m Tc AMLCA were determined in 5 normal dogs by whole body imaging and measuring the radio activity in blood samples at 2,4,8 and 24h after 99m Tc AMLCA injection. The feasibility of imaging of the site of myocardial infarct was determined in 2 of the experimental MI dogs by demonstration of the left ventricle blood pool imaging (LVBPI) with 99m Tc AMLCA and by subsequent imaging of the excised heart. The results showed that the plasma concentration of 99m Tc AMLCA decreased rapidly from 51 5%?5 2% at 2nd hr to 27 3%?3 1% at 4th, 12 3%?1 8% at 8 hr and 5 6%?0 6% at 24th after the injection. The LVBPI in one MI dog showed that the region of the radionuclide concentration of 99m Tc AMLCA corresponded to region of absence of 99m Tc MIBI. The scintigraphy in another MI dog heart slices showed that the region of the radionuclide concentrate of 99m Tc AMLCA corresponded to the region of triphenyltetrazolium chloride (TTC) staining. The measurement of double radionuclides in the double interesting region in the MI dog heart slices indicated that the infarct myocardium uptook 99m Tc AMLCA specifically. These findings suggested that 99m Tc AMLCA scintigraphy might be a new approach for detecting and localizing MI