CLONING OF HUMAN ANGIOPOIETIN-1 GENE AND CONSTRUCTION OF ITS EUKARYOTIC AND PROKARYOTIC EXPRESSION VECTORS / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)1983.
Article
in Chinese
| WPRIM
| ID: wpr-553528
ABSTRACT
Angiopoietin is a novel angiogenesis related molecule. To investigate its roles in tumor angiogenesis and metastasis,angiopoietin 1 gene was obtained from human placental tissue by reverse transcription polymerase chain reaction (RT PCR). The sense, antisense eukaryotic expression vector pcDNA3.1/hangiopoietin1(+), antisense eakaryotic expression vector pcDNA3.1/hangiopoietin1(-), and prokaryotic expression vector pRSETC hangiopoietin1 were successfully constructed by directional cloning the target gene into pcDNA3.1 V5 His C and pRSET C vectors, respectively. This lays good foundation for further study on the roles of angiopoietin 1 in angiogenesis in tumors of the digestive tract.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
1983
Type:
Article
Similar
MEDLINE
...
LILACS
LIS