EXPRESSION AND FUNCTION OF THE ?-ADRENOCEPTORS IN RAT DETRUSOR MUSCLE / 解放军医学杂志

ABSTRACT

To investigate the expression of ? adrenoceptors(? AR) and its possible function in rat detrusor. The relaxation response produced by ? AR subtypes(? 1 , ? 2 , ? 3 AR) agonists or antagonists were examined by means of the in vitro detrusor strip study, and the expression of ? AR subtypes mRNA was investigated in rat detrusor muscle by RT PCR. The selective ? 1 agonist T 0509, ? 2 agonist salbutamol, ? 3 agonist BRL37344A depressed the contractions of the detrusor muscle induced by KCl. The relaxant response to isoproterenol was antagonized by the ? 1 ,? 2 AR antagonists(100?mol/L) and the ? 3 AR antagonists SR59230(1?mol/L).RT PCR, showing that all three subtypes of ? AR subtypes mRNA were expressed in rat detrusor smooth muscles, in which ? 3 AR mRNA occupied 70%. All the ? 1 ,? 2 ,? 3 adrenoceptors participate in mediating relaxation of rat detrusor, among them ? 3 AR is the main one.