EFFECTS OF LPS, TNF-?, IL-1? ON CYCLOOXYGENASE-2 EXPRESSION IN RAT POLYMORPHONUCLEAR LEUKOCYTES / 解放军医学杂志

ABSTRACT

To explore the effects of lipopolysaccharide (LPS), TNF ? and IL 1? on cyclooxygenase 2 (COX 2) expression and prostaglandins (PGs) in rat polymorphonuclear (PMN), measured COX 2 mRNA was determined by reverse transcription polymerase chain reaction and changes in PGs after stimulation of PMN with LPS, TNF ? and IL 1? for 24 hours were observed. The resting PMN were found to express COX 2 mRNA weakly, while it was significantly up regulated after the stimulation of LPS,TNF ?and IL 1?. The level of PGs was increased markedly at the same time. These results suggested that COX 2 might play an important role during inflammatory process involving PMN.