THE CHANGES IN CELLULAR IMMUNITY IN A CANINE MODEL OF GUNSHOT WOUND IN EXTREMITIES IN HOT AND HUMID ENVIRONMENT / 解放军医学杂志

ABSTRACT

Eighteen Chinese mongrel dogs were divided into 3 groups randomly according to the given environment normal environment (NE) group, hot and humid environment (HHE) group, heat acclimatization (HA) group. The dynamic changes in the lymphocyte subsets with CD4 + , CD8 + ,CD11a + ,CD11b + expressions and neutrophil with CD11a and CD11b expressions in peripheral blood were detected post trauma using mAb FACS immuno fluerescence technique. In HHE group, there was a temporary increase (1h) and prolonged decrease (10h) in CD4 + subset, with an increase in CD8 + subset (4h later), resulting in final decrease in CD4 + /CD8 + ratio. In addition, CD11a + T cell decreased 8h later, with a mild change in CD11b + subset. The mechanism is unclear presently. Adaptive training seemed to raise the level of CD4 + and CD11a + lymphocyte subsets and suppress the expression of CD8 + subset. The expression of CD11a and CD11b in NE group increased immediately after injury, presenting a second peak at 4h. The second peak with a higher level was also observed in HHE group, but its duration was shorter than that of the NE group. Heat acclimatization lowered the level of the second peak, though it was remarkably prolonged. Changes in lymphocyte subsets and adhesion molecules of neutrophil suggested that hot and humid environment might suppress the immunity after gunshot injuries, while adaptive exercise could blunt the immunosuppressive response.