Cloning and sequencing of the CTX-M-12 encoding gene of ?-lactamase / 中华传染病杂志

ABSTRACT

Objective To investigate the ESBL gene in a Klebsiella pneumoniae strain isolated from a patient in Huashan Hospital, Shanghai. Methods ESBL gene in the transconjugant from Klebsiella pneumoniae isolate was amplified by PCR. The PCR products were cloned into pHSG398 vector. The antibiotic susceptiblity and ?-lactamases activity of the clone strain were tested; The PCR product was sequenced and analyzed. Results The ESBL gene was identified as CTX-M-12 gene and CTX-M-12 is much more active against cefotaxime than against ceftazidine.Conclusions A clinical isolate of Klebsiella pneumoniae k99-442 isolated from a patient in Huashan Hospital, Shanghai produced ESBL type CTX-M-12 which caused this isolate resistant to most ?-lactams.