THE EFFECT OF HEPATOCYTE GROWTH FACTOR ON SECRETION OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN VASCULAR SMOOTH MUSCLE CELLS AND CARDIOMYOCYTES / 解放军医学杂志

ABSTRACT

The effects of hepatocyte growth factor(HGF) on the secretion of vascular endothelial growth factor(VEGF) in cultured bovine coronary artery endothelial cells(BCAEC), bovine coronary artery smooth muscle cells(BCASMC) and mouse cardiomyocytes were observed in vitro . The concentrations of VEGF in BCAEC control and HGF group at 12h were 10.51?2.90pg/ml and 9.31?2.78pg/ml, respectively. The concentrations of VEGF at 12h in BCASMC control and HGF group were 3.35 and 3.93 times of that at 3h respectively, the concentration of VEGF at 3h and 12h in BCASMC HGF group was 2.06 and 2.42 times respectively, of that in control group. The concentrations of VEGF at 12h in cardiomyocyte control and HGF group were 5.43 and 4.09 times, respectively, of that at 3h, and the concentrations of VEGF at 3h and 12h in cardiomyocyte HGF group was 2.74 and 2.06 times, respectively, of that in control group. These results indicated that BCAEC, BCASMC and mouse cardiomyocytes could secrete VEGF, and HGF could promote VEGF secretion in BCASMC and cardiomyocytes, but not in BCAEC.