EFFECTS OF FREE FATTY ACID ON GENE, PROTEIN EXPRESSION AND TYROSINE PHOSPHOLATION OF LEPTIN RECEPTOR IN RAT ISLET CELLS / 解放军医学杂志

ABSTRACT

Objective The effects of free fatty acid on leptin receptor in rat islet cells were investigated. Methods Islet cells of newborn sprague Dawley rats were incubated with palmitate acid (0 25mmol/L) or oleic acid (0 125mmol/L) for 12 hours, 24 hours and 36 hours respectively. Western blotting was used to assess the protein content of leptin receptor in rat islet cells. The RNA expression level of leptin receptor was assayed by RT PCR. Tyrosine phospholation of leptin receptor was determined by method of immuno precipitation. Results The protein content of leptin receptor in rat islet cells was significantly decreased after being incubated with free fatty acids for 24 and 36 hours compared with the control ( P