Inhibitinon of human telomerase activity by antisense oligonucleotides in heLa cells / 中国药理学通报

ABSTRACT

AIM To study the effect of antisense oligonucleotide s of telomerase on the telomerase activity and the mechanisms of its inhibiting cervical carcinoma HeLa cell line proliferation. METHODS The cytotoxic effect in vitro was determined using the trypan blue dye exclusion assay. The telomerase activity was performed by PCR-TRAP and the flow cytometry analysis was employed to investigate the effects of ASON on the cell cycle and apoptosis. RESULTS There was no significant decrease in cell viability after treated with ASON for 5 days and the ASON with FuGENE6 reduced the telmerase activity compared with the tandem primer and the empty controls. The efficancy depended on the dose of ASON and the time of treatment. ASON did not induce cell apoptisis but increased G 2/M-phase cells. CONCLUSION Antisense oligonucletides of telomerase inhibited the telomerase activity but did not induce apoptosis in HeLa cell lines .Retardation of G 2/M-phase may be the mechanism of inhibiting proliferation in HeLa cell lines.