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The Effect of Estrogen on the DNA Methylation of B Cells in Patients with SLE / 대한류마티스학회지
The Journal of the Korean Rheumatism Association ; : 23-30, 2007.
Article in Korean | WPRIM | ID: wpr-55433
ABSTRACT

OBJECTIVE:

Epigenetics is an important, alternative mechanism of gene regulation that is independent of the nucleotide sequences of DNA. We investigated mRNA levels for DNA methyltransferase-1 (DNMT-1), and the effect of estrogen on the expression of DNMT-1 level in T cells and B cells from patients with systemic lupus erythematosus (SLE) and healthy subjects, and assessed the possible etiological role of DNA methylation in the pathogenesis of SLE.

METHODS:

mRNA levels for DNMT-1 in CD4+ T cells and CD19+ B cells from 37 patients with SLE and 12 healthy controls were examined using RT-PCR. We used specific primer for DNMT-1 and beta actin, The effect of estrogen on the DNA methylation was measured by the mRNA level of DNMT-1 CD4+ T cells and CD19+ B cells treated with 100 nM of 17beta-estradiol for 72 hour.

RESULTS:

The levels of DNMT-1 mRNA were significantly lower in CD4+ T cells and CD19+ B cells from SLE patients compared with healthy controls. We observed the suppression of the levels of DNMT-1 mRNA by stimulated with estrogen in patients with SLE patients, especially in CD19+B cells. DNA hypomethylation of B cells was tend to be correlated with the level of anti-ds DNA antibody without statistical significance (r=-0.43, p=0.3).

CONCLUSION:

Our observations suggest that suppression of DNMT-1 by estrogen in B cells from patients with SLE might be related to the pathogenesis of SLE. Epigenetic studies may provide clues for developing new treatment strategies of SLE.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA / RNA, Messenger / B-Lymphocytes / Base Sequence / T-Lymphocytes / Actins / DNA Methylation / Estrogens / Epigenomics / Lupus Erythematosus, Systemic Limits: Humans Language: Korean Journal: The Journal of the Korean Rheumatism Association Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: DNA / RNA, Messenger / B-Lymphocytes / Base Sequence / T-Lymphocytes / Actins / DNA Methylation / Estrogens / Epigenomics / Lupus Erythematosus, Systemic Limits: Humans Language: Korean Journal: The Journal of the Korean Rheumatism Association Year: 2007 Type: Article