Preliminary study on cell-mediated immunity induced by VP_1 gene vaccine against coxsackievirus B_2 / 中国实用内科杂志
Chinese Journal of Practical Internal Medicine
;
(12)2000.
Article
in Chinese
| WPRIM
| ID: wpr-554408
ABSTRACT
Objective To construct a novel VP 1 gene vaccine against coxsackievirus B 2 and to evaluate the effect of the cell-mediated immunity induced by it.Methods The immunodominant capsid protein VP 1 gene of CVB 2 was amplified by reverse transcript polymerase chain reaction (RT-PCR) and pcDNA 3-CVB 2VP 1was constructed by molecular cloning.The cytotoxic T lymphocyte (CTL) activity was measured by standard 51Cr-release cytotoxicity assay eight weeks after BALB/c mice were immuned by pcDNA 3-CVB 2VP 1.Results The eukaryotic expression vector was pcDNA 3 and subcloning fragment was CVB 2VP 1.The CTL activity of pcDNA 3-CVB 2VP 1 group was higher than that of the control (P
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Practical Internal Medicine
Year:
2000
Type:
Article
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