Your browser doesn't support javascript.
loading
COMPARISON OF DENDRITIC CELL DIFFERENTIATION AND MATURATION IN VITRO IN PERIPHERAL BLOOD MONONUCLEAR CELL CULTURES OF NORMAL SUBJECTS AND PATIENTS WITH GASTROINTESTINAL CARCINOMA / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army ; (12)2001.
Article in Chinese | WPRIM | ID: wpr-554479
ABSTRACT
Objective To explore whether gastrointestinal carcinoma could influence differentiation and maturation of dentritic cell (DC) in cultured peripheral blood mononuclear cells (PBMC). Methods PBMC of peripheral blood obtained either from healthy individuals or gastrointestinal carcinoma patients were incubated to induce into DC with the aid of addition of GM-CSF, IL-4 and TNF-?. The percentage of DC and expression rate of markers CD83, CD1a were determined by flow cytometric analysis. Results The quantity of PBMC isolated from gastrointestinal carcinoma patients was less than that from healthy individuals with same volume of blood. DC could be induced from PBMC of gastrointestinal carcinoma patients, and they could express B7 costimulatory molecules as those of healthy individuals. But the expression rates of CD83, CD1a were significantly different in two groups. Conclusion The quantity of PBMC and their ability to differentiate into DC seemed to be decreased in gastrointestinal carcinoma patients.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Medical Journal of Chinese People's Liberation Army Year: 2001 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Medical Journal of Chinese People's Liberation Army Year: 2001 Type: Article