Expression of BUB1 gene in trophoblastic cells was depressed by microRNA / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)1987.
Article
in Chinese
| WPRIM
| ID: wpr-554648
ABSTRACT
Objective:
To discuss the depressed expression of BUB1 gene in trophoblastic cells by microRNA.Methods:
Endogenous BUB1 mRNA level was compared by using quantitative real-time PCR before and after transfected with microRNA plasmid; and the Bub1 protein level was compared by western blot.Results:
Ratio of BUB1 mRNA/GAPDH mRNA of control group was 0.1960?0.0688,and after transfected with microRNA recombinant plasmid was 0.1968?0.0689 and 0.1962?0.0670 respectively.Statistical study of samples showed they have no instinct different;otherwise the protein level was significantly decreased after transfected with microRNA plamid.Conclusion:
This study established microRNA can control gene expression in mammalian cells at translation level,it is a good foundation to study on the control of gene expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
1987
Type:
Article
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