Study on the role of the chimeric hsp70/CD80 DNA vaccine for treating infection of Mycobacterium tuberculosis / 中华传染病杂志

ABSTRACT

Objective To study the role of the chimeric hsp70/CD80 DNA vaccine for treatment for TB. Methods C57BL/6N mice challenged with H37Rv were immunized with the chimeric hsp70/CD80 DNA vaccine, hsp70 DNA vaccine and BCG in order to compare the therapeutic role of these vaccines. Results The levels of interferon ? (IFN-?) in the serum of mice in hsp70/CD80 group (1336.98?129.64) pg/ml was significantly higher than in group BCG (121.54?56.39) pg/ml, pcDNA3 (192.00?64.36) pg/ml and pcDNA3 hsp70 (542.33?99.77) pg/ml. The growth of Mycobacterium tuberculosis in live and spleen were inhibited significantly in hsp70/CD80 vaccinated mice. Conclusions The chimeric hsp70/CD80 DNA vaccine was more efficient than BCG and hsp70 DNA vaccine alone in treating TB.