Detection and identification of intestinal pathogenic bacteria by oligonucleotide array / 中华传染病杂志

ABSTRACT

Objective To detect the intestinal pathogenic bacteria quickly and accurately from water. Methods An experimental procedure is set up using the gene chip technology to detect and identify common intestinal pathogenic bacteria from water. Target gene was amplified and hybridized with prepared gene chip.Results 143 strains of bacteria in pure culture belong to 9 genera are successfully discriminated under comparatively same condition and a series of specific hybridization maps corresponding to each kinds of bacteria are obtained.Conclusions Salmonella spp., Shigella spp., Staphylococcus spp., Proteus spp., Escherichia coli, Yersinia enterocolitica, Listeria monocytogenes and Vibrio parahaemolyticus are detected and identified by the technology of gene chip. The accuracy, range, and discriminatory power of the assay can be continually improved by adding further oligonueleotides to the arrays without significantly increasing complexity or cost.