The effect of shortly inhaled isoflurane or enflurane on c-fos gene expression of limbic system in rat / 中国药理学通报

ABSTRACT

AIMTo investigate the effect of shor tly inhaled isoflurane or isoflurane on c-fos gene expression of limbic system in rat. METHODS18 SD male rats weighing 200～250 g were randomi zed into three groups, control group, isoflurane group, enflurane group.The anim als in enflurane group or isoflurane group breathed 2% enflurane or 2% isofluran e till righing reflex nearly disappear.The animals in control group underwent th e same experimental steps except inhalation of anesthesia.The effect of shortly inhaled isoflurane or enflurane on c-fos gene in limbic system was observed wit h Fos immunohistochemical staining technique. RESULTNumber of FL I positive neurons of the 10 nucleus was increased significantly in limbic syst em by isoflurane, but enflurane only influenced FLI positive neurons expression of 8 out of the 10 nucleus. CONCLUSIONNuclei with significant ch ange of c-fos expression might be related to isoflurane or enflurane anesthes ia induction.