Enrichment and purification process of total flavonoids from Scutellaria baicalensis with macroporous resins / 第二军医大学学报

ABSTRACT

Objective: To determine the suitable condition for enrichment and purification of total flavonoids from Scutel-laria baicalensis with macroporous resins and to select the optimal parameters. Methods: Using the total flavonoids as the standard, the optimum macroporous resins were selected. Sample concentration, adsorption duration and alcohol concentration were investigated by orthogonal experiment design of form L9(34). Results: The 1300 resin was the suitable one for enriching and purifying total flavonoids. The optimum conditions Were as followsadsorption time at 40 min, sample concentration at 4. 00 mg/ml (pH 5. 0) ,and 30% alcohol as the elution reagent. Conclusion: 1300 resin can be used to enrich and purify the total flavonoids of Scutellaria baicalensis, which can increase the rate of enrichment.