Replication of collagen induced arthritis in C57BL/6 mice / 解放军医学杂志

ABSTRACT

Objective To replicate a model of type Ⅱ collagen (C Ⅱ) induced arthritis in C57BL/6 mice. Methods C57BL/6 mice were immunized by intradermal injection at the base of the tail with chick type Ⅱ collagen and complete Freund adjuvant emulsion, followed by another injection 21 days later. Manifestations of joint ailment, pathological examination, and T cell subtypes detected by FCM were observed. Results In comparison with control mice, the C57BL/6 mice developed CIA with high incidence (70%) and severity after immunization. Hyperplasia of the synovium and inflammatory infiltration were observed. The percentage of Th1 cells in the peripheral blood of the mice was significantly increased in the peripheral mononuclear cells (PBMC). Conclusion The CIA model of C57BL/6 mice and the platform its study were successfully established.