Angiogenesis stimulated by platelet-derived growth factor in aged mice / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1985.
Article
in Chinese
| WPRIM
| ID: wpr-555150
ABSTRACT
Objective:
To investigate the role of platelet-derived growth factor(PDGF)-AB on angiogenensis in aged mice.Methods:
Cardiac microvascular endothelial cells(CMEC)from the hearts of young adult and old mice were cultured.Expression of PDGF-BB and PDGF-AA was detected by RT-PCR.The migration of CMEC was determined with ChemoTX Chamber.Ear angiogenesis model was made in mice.Blood flow in neo-microvessels and collateral vessels was measured with Laser Doppler.Then biotin-labeled Dextran-lysine was injected into the mice through cardiac puncture to label vessels.Ears were cut and immunohistochemistry was carried out by ABC method.Results:
Both PDGF-BB and PDGF-AA were highly ex-pressed in CMEC of young adult mice;expression of PDGF-BB was not detected in aged mice.PDGF-AB reverted the levels of PDGF-BB in aged CMEC to the levels of young adult mice.Migration rate of CMEC in aged mice was significantly increased after stimulation by PDGF-AB(P
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1985
Type:
Article
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