Effects of islet B cells of rats on the differentiation of T-helper lymphocytes / 中国实用内科杂志

ABSTRACT

Objective To investigate the effects of islet B cells of rat on the differentiation of T-helper lymphocytes and its potential mechanism.Methods Forty male SD rats were equally divided into five groups.Group-Arender diabetes by injection of streptozotocin (STZ);Group-BOVA sensitized and then OVA challenged to render asthma;Group-Crender diabetes and then OVA sensitized,and challenged;Group-Drender diabetes,and then insulin subcutaneous injection,OVA sensitized,and challenged;Group-Enondiabetes,nonsensitized,only OVA challenged.Bronchoalveolar lavage (BAL) was performed in rats from each group 24h after challenged and BAL fluid was used to detect IFN-? and IL-4.Blood glucose was estimated,total and differential leukocyte counts were carried out in blood samples and C-peptide concentration was tested in serum.Results The C-peptide concentration in serum was decreased significantly,while INF-? expression was higher,and IL-4 expression was markedly lower in BAL fluid in both group-A and group-C when compared with that of controls,respectively.Compared with group-C,IL-4 expression was increased and INF-? were significantly lower,respectively in BAL fluid of group-D after insulin subcutaneous injection.Conclusion Islet B cells of rat might modulate the differentiation of T-helper lymphocyte.