The effects of human Th2 cytokine on expression of intercellular adhesive molecules on bovin aortic endothelial cells / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-555179
ABSTRACT
Objective To investigate the protective effects of Th2 cytokine (hIL-4, hIL-10, hIL-13) on the bovine aortic endothelial cells(BAECs) stimulated by tumor necrosis factor alpher(TNF-?). Methods BAECs were activated by TNF-?(4ng/ml), and a varied dose of Th2 cytokine(2, 5, 10, 20 and 40ng/ml) was used to incubate BAECs for 2h before stimulation with TNF-?, and then co-incubate for 6h or 18h. The expression of cellular adhesive molecules on BAECs was examined by the cellular enzyme-linked immunoabsorbent assay (Cell-ELISA), and BAECs viability was determined by MTT method. Results BAECs pre-treated with Th2 cytokine could down-regulate the expression of E-selectin and ICAM-1 induced by TNF-? in a dose-dependent manner(P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
2001
Type:
Article
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