The suppressive effect of bone marrow stromal cells modified by CTLA4Ig-gene on lymphocyte activation / 解放军医学杂志

ABSTRACT

Objective To investigate the suppressive effect of bone marrow stromal cells modified by CTLA4Ig-gene on lymphocyte activation. Methods The expressions of CTLA4Ig-gene at transcription level and protein level were assessed by RT-PCR, immunohistochemical techniques and Dot-ELISA. The suppressive effect of CTLA4Ig on lymphocyte activation was investigated in MLR system through MTT and ELISA which quantified IL-2 level. Results The transcription of CTLA4Ig-gene could be detected by RT-PCR in the transfected group and in the passage number two. Immunohistochemistry indicated that CTLA4Ig expression was diffusely located in the cytoplasm of BMSCs. Dot-ELISA could detect the expressed CTA4Ig in the culture medium at 12h and even in culture medium of the passage number l after transfection. MLR system showed that CTLA4Ig had significant inhibition on lymphocyte proliferation and IL-2 secretion. Conclusion Ad-CTLA4Ig could transfect BMSCs efficiently. Morever, CTLA4Ig can suppress lymphocyte activation and IL-2 production.