Application of optical coherence tomography technique in the research of rat retinal light damage / 第三军医大学学报

ABSTRACT

Objective To evaluate the application of optical coherence tomography (OCT) in the rat retinal light damage. Methods Albino Sprague-Dawley (SD) rats (5-8 weeks of age) were exposed to 1 000-1 400 lux of diffuse, cool, white, fluorescent light for 2, 5, and 7 d. OCT image analysis and histological measurements of the retinal thickness were performed. Animals were then sacrificed and the measured results were compared with those by histological examination. Results The sensory retinal thickness of the retina in the rats thinned progressively as the retinal degeneration was in progress. The sensory retinal thickness measured by OCT [the corresponding thickness was (179.11?12.01)?m, (159.27?12.81)?m, and (133.67?11.43)?m, respectively] was well correlated with that measured by histology [the sensory retinal thickness after exposure to the light for 2, 5, and 7 d was (144.26?9.36)?m, (116.16?11.24)?m, and (94.27?10.68)?m, respectively] (r= 0.995, P