Epigallocatechin-3-gallate Inhibits the Expression of Adhesion Molecules by Blocking Nuclear Factor Kappa B Signaling in Intestinal Epithelial Cells
Intestinal Research
;
: 261-267, 2013.
Article
in English
| WPRIM
| ID: wpr-55530
ABSTRACT
BACKGROUND/AIMS:
Epigallocatechin-3-gallate (EGCG) is the main polyphenol in green tea and has anti-inflammatory and anti-oxidative effects. The aim of this study was to determine the impact of EGCG on the expression of adhesion molecules and lipopolysaccharide (LPS)-induced nuclear factor-kappa B (NF-kappaB) signaling in rat intestinal epithelial (RIE) cells.METHODS:
The effect of EGCG on LPS-induced NF-kappaB signaling and expression of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 was examined by reverse transcription polymerase chain reaction, western blotting, immunofluorescence and electrophoretic mobility shift assay.RESULTS:
LPS-induced expression of ICAM-1 and VCAM-1 mRNA was inhibited by EGCG treatment in RIE cells. LPS-induced inhibitor of kappa B alpha degradation and NF-kappaB nuclear translocation were blocked by EGCG in RIE cells. EGCG blocked LPS-induced NF-kappaB DNA-binding activity in RIE cells. The pharmacological NF-kappaB inhibitor Bay11-7082 suppressed the LPS-induced expression of ICAM-1 and VCAM-1 mRNA in RIE cells.CONCLUSIONS:
These results indicate that EGCG inhibits LPS-induced ICAM-1 and VCAM-1 expression by blocking NF-kappaB signaling in intestinal epithelial cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sulfones
/
Tea
/
RNA, Messenger
/
Catechin
/
Blotting, Western
/
Polymerase Chain Reaction
/
NF-kappa B
/
Fluorescent Antibody Technique
/
Intercellular Adhesion Molecule-1
/
Vascular Cell Adhesion Molecule-1
Limits:
Animals
Language:
English
Journal:
Intestinal Research
Year:
2013
Type:
Article
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