Enhancement of NGF-induced neuronal differentiation of PC12 cells by co-culturing with primary neurons and glia cells / 解放军医学杂志

ABSTRACT

Objective To establish an in vitro cell co-culture model that simulates the transplantation environment in vivo ,and to investigate the effect of primary cells from neonatal cortex on the differentiation of PC12 cells. Methods PC12 cells were transfected with pEGFP-N1 vector to express green fluorescent protein (GFP) stably,then these cells were transplanted into primary neurons and glial cells from neonatal cortex. Cultures with PC12 cells alone constituted the control group. Nerve growth factor (NGF) was added into all the other cultures to induce neuronal differentiation of the PC12 cells. Neurite outgrowth of the neuronal PC12 cells was observed with laser confocal microscopy. Results Comparing with the control group,the PC12 cells co-cultured with primary neurons and glial cells showed higher differentiation rate (57.13% to 67.77%,P